Biotech Primer Institute

How Diagnostic Tests Work: Protein-Based Diagnostics

19 STUDENTS ENROLLED

Course Availability: Unlimited views for 72 hours from time of purchase
Course Running Time:
40 minutes | Assessment: 20 minutes
Course Material: Downloadable PDF of all presented slides
Preview Now: Defining Protein Based Diagnostics

How Diagnostic Tests Work: Protein-Based Diagnostics focuses on antibodies: what they are, where they come from and how they work. Various antibody-based diagnostics, such as sandwich and bead immunoassays, multiplexed assays, lateral flow assays and protein chromatography are explained in detail. Develop an understanding of these diverse tools with this course. Running time: 40 minutes | Assessment: 20 minutes

Defining Protein Based Diagnostics
At the end of this section you should be able to:
1. List the various protein-based diagnostics
2. Define biomarker and explain their importance in molecular diagnostics

Antibody Technology
At the end of this section you should be able to:
1. Draw the structure and explain the function of antibodies
2. Discuss how properties of an antibody can be exploited for diagnostic use

Enzyme-Linked Immunosorbent Assays (ELISA) Technology
At the end of this section you should be able to:
1. Explain how the ELISA technology works
2. Relate how an ELISA is used in diagnostic applications
3. Interpret the information received from an ELISA diagnostic

Bead Immunoassay Technology
At the end of this section you should be able to:
1. Explain how the ELISA is adapted to high throughput techniques, including multiplexed bead assays
2. Explain how bead immunoassays work
3. Describe how bead immunoassay is used in diagnostic applications
4. Interpret the information received from an immunoassay diagnostic

Lateral Flow Assay Technology
At the end of this section you will be able to: Lateral Flow
1. Define the term Immunochromatographic
2. Explain how lateral flow assays work
3. Describe how lateral flow assays are used in diagnostic applications
4. Interpret the information received from an lateral flow assay diagnostic

Chromatography Technology
At the end of this section you will be able to:
1. Explain how column chromatography works
2. Describe how column chromatography can be used in diagnostic applications
3. Interpret the information received from a chromatography diagnostic

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